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KMID : 0363320040250020268
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Journal of Korean Oriental Internal Medicine
2004 Volume.25 No. 2 p.268 ~ p.276
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Peroxynitrite Scavenging Mechanism of Psoralea corylifolia
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Jung Ji-Chun
Jang Yong-Suk
Min Gun-Woo
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Abstract
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Objective : Peroxynitrite (ONOO-), formed from the reaction of O_2- and NO, is a cytotoxic species that can oxidize several cellular components such as proteins, lipids and DNA. It has been implicated in the aging process and age-related disease such as Alzheimer’s disease, rheumatoid arthritis, cancer and atherosclerosis. The aim of this study was to investigate scavenging activities of ONOO- and its precursors. NO and O_2- and its scavenging mechanism using fluorescent probes, DCFDA, DAF-2 and DHR 123..
Methods : Psoralea corylifolia was crushed. The crushed Psoralea corylifolia was extracted 3 times, each time with 3 volumes of methyl alcohol at 60{circ}C for 24 h. The extract was filtered and evaporated under reduced pressured using a rotary evaporator to yield 16g. This was done to investigate scavenging activities of ONOO-, NO, O_2- and its scavenging mechanism using fluorescent probes, DCFDA, DAF-2 and DHR 123.
Results : After Psoralea corylifolia was added authentic ONOO-,;{cdot};O_2- and NO was markedly scavenged. Also, ONOO- induced by O_2- and NO (these derived from SIN-1) was inhibited. The data showed a decrease in ONOO- mediated nitration of tyrosine through electron donation after Psoralea corylifolia was added. Data showed a dose-dependent correlation with inhibition of nitration of bovine serum albumin induced by ONOO-, Furtheremore, LPS-induced ROS and RNS generation was blocked.
Conclusions: These results suggest potential for use of Psoralea corylifolia as an effective ONOO- scavenger to counter the aging process and age-related diseases.
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KEYWORD
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Peroxynitrite, Scavenging Mechanism, Psoralea corylifolia
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